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Molecular Biology Workshop
February 19 - 23, 2018
DESCRIPTION: This intense 5 day workshop will focus on a myriad of different techniques used in the molecular manipulation of DNA (general cloning, transformation, silica kits, pI kits, PCR, qPCR), RNA (isolation, reverse transcription) and protein (SDS-PAGE, 2D gels), as well as lectures that will describe some high throughput technologies such as SNP analysis, and next generation sequencing. Primarily aimed at researchers who are new to the area, familiar but require a quick updating, or theoretically familiar but lacking in practical bench training. Note: That this session will also include hands-on and theoretical content around Next Gen Sequencing (specifically around the use of the Ion Torrent system).
PHILOSOPHY: Whilst molecular techniques have evolved at a blindingly fast rate over the last few decades, the underlying biochemical principles behind the vast majority of them have actually changed little. This workshop therefore combines opportunities to perform the latest, as well as commonly used older techniques, with particular attention to the chemical nuts and bolts behind them. In all, this allows the researcher to not only gain needed practical hands-on familiarity with the techniques, but also achieve a comfortable theoretical level to allow for both (1) that all important skill of troubleshooting, and (2) the often undervalued skill of judging the utility of “tricks” that aim to speed up, or lower costs of a given methodology.